Herein, for the first time, a new generation cysteine modified MoS2(Cys@MoS2) based electrochemical sensor was reported. The electrochemical behaviour of dapagliflozin (DAP) was investigated through differential pulse voltammetry (DPV) on the developed sensor (Cys@MoS2/GCE). The transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), x-ray diffraction spectroscopy (XRD) and x-ray photoelectron spectroscopy (XPS) methods were performed for structural and morphological characterizations of Cys@MoS(2)nanocomposite. On the surface of Cys@MoS2/GCE, an irreversible anodic peak was observed at 1324 mV. Under the optimal conditions, linear calibration curve with two working ranges between 2.0-60.0 mu M and 60.0-110.0 mu M were obtained and limit of detection was found to be 1.6 mu M. The developed sensor was successfully applied to determine the content of DAP in pharmaceutical sample with satisfying recovery results. It is concluded that Cys@MoS2/GCE is a reliable, easy to apply and cost-effective sensor for the routine DAP analysis in pharmaceutical samples.