The beliefs and attitudes toward sexuality during pregnancy, as well as anatomical, physiological, and spiritual changes that women experience, affect sexual life in this period. This study aims to develop a measurement tool to determine the attitudes of women with pregnancy and men whose partners were pregnant toward sexuality during pregnancy. As a result of the psychometric analysis, a 34-item, 3-factor scale of attitudes toward sexuality during pregnancy was obtained. The Attitude Scale toward Sexuality during Pregnancy was found as a valid and reliable tool in Turkish women with pregnant and their partners. The scale is brief, easy to understand, administer and score, and demonstrates high internal consistency. Another importance of this scale is that it can be used for men. It may prove useful for researchers and clinicians interested in sexuality.