Preparation of a Polypyrrole-Polyvinylsulphonate Composite Film Biosensor for Determination of Phenol Based on Entrapment of Polyphenol Oxidase


ARSLAN H. , ARSLAN F.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.39, no.5, pp.341-345, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.3109/10731199.2011.585616
  • Title of Journal : ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Page Numbers: pp.341-345
  • Keywords: biosensor, amperometry, polypyrrole (PPy), polyvinylsulphonate (PVS), entrapment, interference effect, AMPEROMETRIC BIOSENSOR, ENZYME ELECTRODE, IMMOBILIZATION

Abstract

In this paper, a novel amperometric phenol biosensor with immobilization of polyphenol oxidase (tyrosinase) on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique on the surface of a platinum electrode. The amperometric determination is based on the electrochemical reduction of quinon generated in the enzymatic reaction of phenol. The effects of pH and temperature were investigated and optimum parameters were found to be 8.0 and 30 degrees C, respectively. The linear working range of the electrode was 1.0 X 10(-7) - 5.0 X 10(-6) M. The storage stability and operation stability of the enzyme electrode were also studied.