Dual-drug double-layer electrospun poly(ε-caprolactone)-chitosan/poly(ε-caprolactone)-poly(ethylene oxide) wound dressing incorporating quercetin and tigecycline
Journal of Drug Delivery Science and Technology, cilt.127, 2027 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 127
- Basım Tarihi: 2027
- Doi Numarası: 10.1016/j.jddst.2026.108915
- Dergi Adı: Journal of Drug Delivery Science and Technology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE
- Anahtar Kelimeler: Chitosan, Double-layer wound dressing, Electrospinning, Quercetin, Tigecycline
- Gazi Üniversitesi Adresli: Evet
Özet
This study reports the development and characterization of a dual-drug double-layer electrospun scaffold composed of poly (ε-caprolactone)/poly (ethylene oxide) (PCL–PEO) containing quercetin (QU) and poly (ε-caprolactone)/chitosan (PCL–CH) containing tigecycline (TG), with emphasis on its physicochemical properties, drug-release behavior, antibacterial activity, and initial in vitro cytocompatibility. All membranes exhibited uniform, bead-free fibers, with diameters ranging from 89 ± 51.7 nm (PCL–CH–TG) to 900 ± 81.5 nm (PCL–PEO–QU). QU caused a notable increase in the surface roughness from 187 ± 36 to 245 ± 65 nm, whereas TG led to a clear decrease from 76 ± 38 to 63 ± 9 nm. Surface wettability results showed that the PCL-CH layer was hydrophobic with a contact angle of 119.9°, while the addition of TG decreased the contact angle to 88.5°. The PCL-PEO and PCL-PEO-QU layers were superhydrophilic (contact angle < 0°). The highest water uptake ratio of 93.27% was achieved with the double layer wound dressing material at 48 h. The double-layer wound dressing material exhibited the highest tensile strength of 14.60 MPa and Young's modulus of 43.50 MPa. In vitro drug release studies showed that QU release from the PCL–PEO–QU layer occurred in a sustained manner by 42% at 24 h; however, TG release from the PCL-CH-TG layer reached almost 98.8% within 120 min. The highest antibacterial activity was observed with the PCL–CH–TG layer against S. aureus , resulting in an inhibition zone of up to 22.1 ± 0.2 mm. The cytocompatibility test confirmed that the double layer and PCL-CH-TG wound dressings exhibited non-cytotoxic behavior, with viability exceeding 100%, while the PCL–PEO–QU dropped to 68.6%. Overall, the double-layer system may provide a promising multifunctional electrospun platform combining distinct drug-release characteristics of the constituent layers, antibacterial activity, and favorable physicochemical and cytocompatibility properties.