In this work, di- and mono-halogenofluorobenzene dervatives synthesized and identified by spectroscopic means H-1 NMR and F-1 NMR. Di- and mono-halogenofluorobenzene dervatives were 2-fluoro-3-chlorobromobenzene (1), 1,3-dibromo-2-fluorobenzene (2), 2-fluoro3-iodobromobenzene (3), 2-bromofluorobenzene (4), 2-iodofluorobenzene (5), 2-chlorofluorobenzene (6). The antibacterial and antifungal activities of di- and mono-halogenofluorobenzene derivatives studied against bacteria i.e. Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 6633, Morganella morganii (clinical isolate), Escherichia coli ATCC 27853, Micrococcus flavus (clinical isolate), Candida albicans (clinical isolate). To measure zone diameter, the antibacterial and antifungal activities were measured by Minumum Inhibition Concentration (MIC) method and disc-diffusion method used against to that gram-positive and gram-negative bacteria and fungus. All the bacteria and fungus studied against to antibiotics like ketoconazole, ampicillin, tetracycline, penicillin, chloramphenicol and oxacillin to compare with our chemicals zone diameters.