Simultaneous determination of metronidazole and miconazole in pharmaceutical dosage forms by RP-HPLC


Akay C., Ozkan S., Senturk Z., Cevheroglu S.

FARMACO, vol.57, no.11, pp.953-957, 2002 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 11
  • Publication Date: 2002
  • Doi Number: 10.1016/s0014-827x(02)01296-x
  • Journal Name: FARMACO
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.953-957
  • Keywords: reversed-phase high performance liquid chromatography, metronidazole and miconazole, pharmaceutical dosage forms, PERFORMANCE LIQUID-CHROMATOGRAPHY, DERIVATIVE SPECTROPHOTOMETRY, AMPEROMETRIC DETECTION, CREAMS, PLASMA, METABOLITES, EXTRACTION, NITRATE, DRUGS
  • Gazi University Affiliated: No

Abstract

A reversed-phase high performance liquid chromatography (RP-HPLC) method with UV detection is described for the simultaneous determination of metronidazole and miconazole in pharmaceutical dosage forms. Chromatography was carried out on a Cl 8 reversed-phase column, using a mixture of methanol-water (40 + 60, v/v) as a mobile phase, at a flow rate of 1.0 ml min(-1). Sulfamethoxazole was used as an internal standard and detection was performed using a diode array detector at 254 nm. The method produced linear responses in the concentration ranges 10-70 and 1-20 mug ml(-1) with detection limits 0.33 and 0.27 mug ml(-1) for metronidazole and micanozole, respectively. This procedure was found to be convenient and reproducible for analysis of these drugs in ovule dosage forms. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.