Geraniol, a natural monoterpene, identifications of cytotoxic and genotoxic effects in vitro

MAMUR S.

JOURNAL OF ESSENTIAL OIL RESEARCH, cilt.34, sa.1, ss.54-64, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1080/10412905.2021.1974581
  • Dergi Adı: JOURNAL OF ESSENTIAL OIL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Business Source Elite, Business Source Premier, CAB Abstracts, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.54-64
  • Anahtar Kelimeler: Chromosomal aberrations, comet, geraniol, MTT tetrazolium assay, micronucleus, neutral red uptake cytotoxicity assay, CELL-CYCLE ARREST, PERILLYL ALCOHOL, NEUTRAL RED, COMET ASSAY, DNA-DAMAGE, EXPOSURE, PROLIFERATION, LYMPHOCYTES, TOXICITY, MICRONUCLEI
  • Gazi Üniversitesi Adresli: Evet

Özet

Geraniol (GER) is an acyclic monoterpene alcohol. This study aimed to investigate the cytotoxic and genotoxic effect of GER in human lymphocytes using chromosomal aberrations (CAs) micronucleus (MN) assays and DNA damaging effect using comet assay in isolated human lymphocytes as well as cytotoxic effect in MCF-7 cells using MTT and Neutral red uptake (NRU) assays. Cells were treated with between 4 and 500 mu g/mL of GER. GER significantly reduced the cell viability at 4, 20, 100 and 500 mu g/mL at 48 hours in MCF-7 cells. GER also had a cytotoxic effect by lowering the mitotic index at 20-100 mu g/mL in human lymphocytes. GER significantly induced the frequency of CAs and CA/cell at both treatments. GER did not significant affect the MN frequency. GER significantly increased the comet tail intensity and moment at 50, 100, and 500 mu g/mL. GER exerted cytotoxic and genotoxic effects especially at higher concentrations.