Heat exchangers are used in many applications including chemical, oil, and gas power generation, refrigeration, pharmaceuticals, and food processing. Because of their widespread usage, they have various types to serve at different working conditions. Increasing the performance of heat exchangers has become a very interesting field of study since the efficiency of various industrial and domestic systems depend on them. In this study, a coaxial double tube experimental set-up was prepared, and the effect of using nanohexagonal boron nitride nanofluid as hot working fluid on the heat transfer performance increase was investigated. The experiments were carried out in concurrent flow and counter flow conditions for various hot fluid-flow rates to obtain the heat transfer coefficients. Nanohexagonal boron nitride obtained in powder form was used to prepare the nanofluid by a two-step method. The 4 kg nanofluid containing 2% nanohexagonal boron nitride with 0.5% Triton X-100 as a surfactant in terms of mass ratio was prepared for the experiments. Heat transfer experiments were carried out three times by using the prepared nanohexagonal boron nitride/water nanofluid and pure water as hot fluid to reach more precise results. In the result of this study, the total heat transfer coefficient showed an average improvement of 48.78% for the concurrent flow heat exchanger, while an average improvement of 0.36% was observed in the counter flow conditions compared to the base fluid. This study shows the potential of application of nanohexagonal boron nitride/water nanofluid in heat management applications.