Designing multilayered nanoplatforms for SERS-based detection of genetically modified organisms

Uluok S., Guven B., Eksi H., ÜSTÜNDAĞ Z., TAMER U., BOYACI İ. H.

JOURNAL OF NANOPARTICLE RESEARCH, cilt.17, sa.1, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1007/s11051-014-2849-5
  • Dergi Adı: JOURNAL OF NANOPARTICLE RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Multilayered platform, Gold nanoparticles, Genetically modified organisms (GMOs), Surface-enhanced Raman spectroscopy (SERS), Nanobiotechnology, SELF-ASSEMBLED MONOLAYERS, GOLD NANOPARTICLES, ELECTROCHEMICAL DETECTION, DNA DETECTION, DENDRIMERS, SPECTROSCOPY, BIOSENSORS, PCR
  • Gazi Üniversitesi Adresli: Evet

Özet

In this study, the multilayered surface-enhanced Raman spectroscopy (SERS) platforms were developed for the analysis of genetically modified organisms (GMOs). For this purpose, two molecules [11-mercaptoundecanoic acid (11-MUA) and 2-mercaptoethylamine (2-MEA)] were attached with Aurod and Auspherical nanoparticles to form multilayered constructions on the gold (Au)(slide) surface. The best multilayered platform structure was chosen depending on SERS enhancement, and this surface was characterised with atomic force microscopy (AFM) and attenuated total reflectance Fourier transform infrared spectroscopy. After the optimum multilayered SERS platform and nanoparticle interaction was identified, the oligonucleotides on the Aurod nanoparticles and Au-slide were combined to determine target concentrations from the 5,5 '-dithiobis (2-nitrobenzoic acid) (DTNB) signals using SERS. The correlation between the SERS intensities for DTNB and target concentrations was found to be linear within a range of 10 pM to 1 mu M, and with a detection limit of 34 fM. The selectivity and specificity of the developed sandwich assay were tested using negative and positive controls, and nonsense and real sample studies. The obtained results showed that the multilayered SERS sandwich method allows for sensitive, selective, and specific detection of oligonucleotide sequences.