Akademik Veri Yönetim Sistemi
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, cilt.7, sa.10, ss.10205-10214, 2012 (SCI-Expanded)
In this study, a novel a carbon paste electrode using the salt form of polyaniline (pani)-silicon dioxide composite sensitive to glucose, was prepared. Glucose oxidase enzyme was immobilized to carbon paste electrode by cross-linking with glutaraldehyde. Determination of glucose was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 degrees C, respectively. The linear working range of the electrode was 5.0 x 10(-6) - 1.0 x 10(-3) M, R-2 =0.997. The storage stability and operation stability of the enzyme electrode were also studied. The results showed that 98.4 % of the response current was retained after 16 activity assays. The prepared glucose biosensor retained 37 % of initial activity after 70 days when stored in 0.1 M phosphate buffer solution at 4 degrees C.