The relationship between clinical course of novel coronavirus (2019-nCoV/SARS-CoV-2) infection with ACE2 and TMPRSS2 expression and polymorphisms

Tuğ E., Bozdayı G., Fidan I., Yıldırım F., Güzel Tunçcan Ö., Lale Z., ...Daha Fazla

24th Annual Conference of the European Society for Clinical Virology, Manchester, İngiltere, 7 - 10 Eylül 2022, ss.186

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Manchester
  • Basıldığı Ülke: İngiltere
  • Sayfa Sayıları: ss.186
  • Gazi Üniversitesi Adresli: Evet

Özet

Aim: The spike (S) protein of SARS-CoV-2, and Angiotensin-Converting Enzyme 2 (ACE2) and Transmembrane Protease Serine 2 (TMPRSS2) genetic variations may act as a barrier in infection or to determine susceptibility to COVID-19 infection. In this context, we investigated the relationship between the expression patterns and polymorphisms of the ACE2 and TMPRSS2 receptor genes associated with COVID-19 and the clinical course of COVID-19 infection.

Methods: ACE2 and TMPRSS2 expressions were determined using the One-Run Real-Time Quantitative Polymerase Chain Reaction (RT-Q-PCR) kit. Genotypic distributions of single nucleotide polymorphisms of ACE2 rs714205, rs73635825, rs2285666, rs1978124, and TMPRSS2 rs8134378, rs2070788, rs7364083, rs13052975, rs9974589 were obtained RT-PCR.

Results: The expression of ACE2 and TMPRSS2 was different between SARS-CoV-2 positive and negative groups. ACE2 and TMPRSS2 expressions of SARS-CoV-2 positive patients treated in the intensive care unit were higher than the control group and other patient groups. ACE2 rs714205GG genotype and G-allele showed significant differences in the SARS-CoV-2 positive asymptomatic group. A significant correlation was found between TMPRSS2 rs8134378GA, rs2070788GA, rs7364083GA and rs9974589AC genotypes and SARS-CoV-2 positivity. The rs1978124 C-allele and rs8134378 A-allele were significant in the SARS-CoV-2 positive symptomatic group. TMPRSS2 rs2070788GA was different in all groups from the control group.

Conclusions: ACE2 rs714205GG may be protective from COVID-19 infection, and TMPRSS2 rs2070788GA, rs8134378GA and rs9974589AC may be genotypes associated with infection susceptibility. In conclusion, identifying the relationship between host genetic variants and COVID-19 susceptibility will contribute to further studies that will enable new vaccines and potential therapeutic approaches to be applicable.