In this study, a new film electrode for determination of hydrogen peroxide was developed. Polypyrrole-poly (sodium-4-styrenesulphonate) film have been prepared on the platinum electrode by the electropolymerization of pyrrole in the presence of poly(sodium-4styrenesulphonate). Optimum synthesis/preparation/formation of polypyrrole-poly(sodium-4styrenesulphonate) film was determined. Oxidation of the hydrogen peroxide was examined at a different potential. Therefore, four different potentials (0.4, 0.5, 0.6, and 0.7 V) were determined. Also, concentration of pyrrole and poly(sodium-4-styrenesulphonate) were examined. The sensitivities of electrodes to hydrogen peroxide were determined to clarify the role of PSS concentration. Three different PSS concentrations (0.05, 0.1, 0.15 M) were examined. The effect of the number of cycles in their sensitivity to hydrogen peroxide is investigated. Morphology of electrodes was qualified by means of SEM. Free enzyme work was performed using the prepared film electrode and glucose oxidase. Linear increases were observed in the amperometric response currents of the electrode versus increasing glucose concentrations. It was shown that this film can be used for hydrogen peroxide producing enzymes.