It is important that health personnel have extensive and adequate knowledge and practice regarding forensic evidence. This article describes the knowledge and practices of health personnel, who work in emergency rooms and health centers, regarding forensic evidence. The health personnel in a city in Central Anatolia, Turkey, constitute the population of this descriptive study and 233 personnel constitute its sample. It was determined that 31.3% stated that the practices of forensic evidence collection are inadequate. It was determined that average knowledge scores of health personnel with respect to forensic evidence are 23.5 +/- 7.28 of 40. It was found that there was a statistically significant difference between knowledge scores on the subject of forensic with respect to duty (p = 0.005), level of education (p = 0.005), and institution of health personnel (p = 0.015). It was determined that the scores of the health personnel, who work in emergency services and health centers, on the subject of forensic evidence, are not at a desirable level.