Indoleamine-2,3-dioxygenase-related anti-inflammatory effects of 3-aminobenzamide and infliximab in experimental colitis

Menekse E., Aydin S., Aydin O. U., Sahin D., Akca G., Eroglu A., ...Daha Fazla

REVISTA DA ASSOCIACAO MEDICA BRASILEIRA, cilt.68, sa.3, ss.362-366, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1590/1806-9282.20210974
  • Dergi Adı: REVISTA DA ASSOCIACAO MEDICA BRASILEIRA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.362-366
  • Anahtar Kelimeler: Inflammatory bowel disease, Ulcerative colitis, 3-Aminobenzamide, Infliximab, Indoleamine 2,3-dioxygenase, TRYPTOPHAN, EXPRESSION
  • Gazi Üniversitesi Adresli: Evet

Özet

OBJECTIVE: This study aimed to investigate the presence of indoleamine-2,3-dioxygenase and bacterial translocation after the administration of 3-aminobenzamide and infliximab in the TNBS modelofratcolitis.
METHODS: The study group was divided into five categories as follows: group 1: (control), group 2: colitis+saline, group 3: colitis+3-aminobenzamide, group 4: colitis+infliximab, and group 5: colitis+3-aminobenzamide+infliximab. Intestinal mesenteric cultures were incubated on specific agar media plates under aerobic and anaerobic conditions, bacterial translocation was evaluated and assessed as colony-forming units per gram of tissue. Colonic tissue samples were evaluated by Western blotting method to detect the presence of indoleamine-2,3-dioxygenase. 

RESULTS: The results obtained were as follows: group 1: normal gut flora; group 2: eight of nine samples had bacterial translocation, of which six of them had positive indoleamine-2,3-dioxygenase protein; group 3: five of nine samples had bacterial translocation, of which seven of them had positive indoleamine-2,3-dioxygenase; group 4: three of nine samples had bacterial translocation, of which seven of them had positive indoleamine- 2,3-dioxygenase; and group 5: only one sample had exact indoleamine-2,3-dioxygenase protein.