Effect of autoclaving process on ion release from base metal alloy into saline was investigated in this study. Alloy specimens were divided into two groups. First group samples were not autoclaved before testing; the second group samples were subjected to 150 degrees C for 60 min. After 7 days, elements released into solution were measured by atomic absorption spectrometry. ANOVA and Tukey test (alpha=0.05) were used to compare the mass released between groups. Autoclaving process caused a significant decrease on mass released compared to non-autoclaved group. This study indicates the importance of cleaning protocols on element release studies, which can affect the results.