New supports for use of Glucose Oxidase enzyme as biocatalysis


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Sarı N. , Bağcacıer Z. N. , Kurnaz Yetim N., Hasanoğlu Özkan E. , Nartop D.

W. A. Science, Engineering and Technology, cilt.78, ss.949-953, 2013 (Diğer Kurumların Hakemli Dergileri)

  • Cilt numarası: 78
  • Basım Tarihi: 2013
  • Dergi Adı: W. A. Science, Engineering and Technology
  • Sayfa Sayıları: ss.949-953

Özet

Nanoparticles modified polystyrenes with some Furfural derivatives were synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on there. The characteristics of the immobilized glucose oxidase (APS-FurH)-GOx, (APS-FurCH3)-GOx, (APS-FurNO2)-GOx enzyme showed except one of them. The insertion of stabile (SchFur-NO2) spacers between polystyrene backbone and the immobilized GOx, (APS-Fur-SchNO2GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4, enzyme showed except one of them. The insertion of stabile (SchFur-NO2) spacers between polystyrene backbone and the immobilized GOx, (APS-Fur-SchNO2GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4.