An amperometric biosensor for L-glutamate determination prepared from L-glutamate oxidase immobilized in polypyrrole-polyvinylsulphonate film

Simsek S., Aynaci E., ARSLAN F.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.44, no.1, pp.356-361, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.3109/21691401.2014.951723
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.356-361
  • Keywords: biosensor, L-Glutamate, L-glutamate oxidase, polypyrrole, polyvinylsulphonate, COMPOSITE FILM, FOOD, ELECTRODE, ACID, DEHYDROGENASE, MEMBRANE, CHOLINE
  • Gazi University Affiliated: Yes


In this paper, a novel amperometric L-glutamate (Glu) biosensor with immobilization of L-glutamate oxidase (L-GlOx) on polypyrrole-polyvinylsulphonate (PPy-PVS) film has been successfully developed. L-GlOx enzyme was immobilized on PPy-PVS film by cross-linking with glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of Glu was carried out by oxidation of enzymatically produced H2O2 at 0.3 V versus Ag/AgCl. The optimum pH and temperature parameters were found to be 9.0 and 55 degrees C, respectively. There were three linear parts in the regions between 1.0 x 10(- 9) and 1.0 x 10(- 8) M (R-2 = 0.847), 5.0 x 10(- 8) and 5.0 x 10(- 7) M (R-2 = 0.997), 5.0 x 10(- 7) and 5.0 x 10(- 5) M (R-2 = 0.994). Storage stability, operation stability of the enzyme electrode were also studied.