Akademik Veri Yönetim Sistemi
II. International Agricultural, Biological & Life Science Conference, Edirne, Türkiye, 1 - 03 Eylül 2020, ss.252-258
In this study, the antibiofilm efficacy of the Lactococcus lactis subsp. lactis LL27 strain isolated from raw milk and identified as nisin producer, against Salmonella Typhimurium and Listeria monocytogenes biofilms were investigated. In the research primarily, autoaggregation capabilities of each strain were determined. The strain with the highest autoaggregation ability was determined as L. monocytogenes ATCC7644 (0.677), followed by S. Typhimurium 14028 (0.55) and L. lactis subsp. lactis LL27 (0.184). In the cocultures of L. monocytogenes ATCC7644 and S. Typhimurium 14028 treated separately with L. lactis subsp. lactis LL27, coaggregation rates (average 0.46) were found close to the autoaggregation rates of the aforementioned pathogens. However, these rates were found to be statistically significant (p <0.05) higher than the autoaggregation rate of L. lactis subsp. lactis LL27 strain. Biofilm experiments were started, as this indicates that the nisin producer strain of L. lactis subsp. lactis LL27 may affect the formation of biofilms by reducing the autoaggregation ability of the pathogens in question. Salmonella Typhimurium 14028 biofilm capacity statistically significant increase (p <0.05) in the cocultures of L. lactis subsp. lactis LL27 + L. monocytogenes ATCC7644 and L. lactissubsp. lactis LL27 + S.Typhimurium 14028. The most
interesting result in the study was the statistically significant decrease (p <0.05) in biofilm production capacity on the 3rd day in L. monocytogenes ATCC7644 and S. Typhimurium 14028 cocultures. These data indicate that direct use of starter culture strains in the fight against biofilm structures of pathogens may promote biofilm production, as opposed to the antagonistic effect expected.