Screening Study on Serine Protease Inhibitory Activity of 10 Plant Species

GÜNBATAN T., DİLMAÇ E., GÖKBULUT A., Sucu M., GÜRBÜZ İ.

Chemistry and Biodiversity, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1002/cbdv.202402776
  • Dergi Adı: Chemistry and Biodiversity
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: antioxidants, chymotrypsin, enzymes, liquid chromatography, trypsin
  • Gazi Üniversitesi Adresli: Evet

Özet

In this research, in vitro serine protease inhibitory activity of 10 plant species was evaluated, and extracts that showed strong activity were analyzed through high-performance liquid chromatography (HPLC). Rhododendron caucasicum Pall. (leaf) and Potentilla reptans L. were found to have the highest chymotrypsin inhibitory activities (83.77% and 82.01% inhibition). The highest trypsin inhibitory activity was observed in R. caucasicum (flower) (82.86% inhibition), followed by Cruciata laevipes Opiz (82.22% inhibition). Extracts showing potent enzyme inhibition were fractioned and subjected to activity tests. The highest chymotrypsin inhibitory activity was observed in the n-hexane fraction of P. reptans (92.90% inhibition). In comparison, the highest trypsin inhibitory activity was found in the ethyl acetate fraction of Lythrum salicaria L. (89.81% inhibition). HPLC studies determined that the 80% ethanol extract of P. reptans contained chlorogenic acid. The screened plants were generally rich in phenol and flavonoid content and showed strong antioxidant activity.