Polarographic determination of anticancer drug vitamin K-3 with direct and indirect methods/application to serum and K-3 injection solution


Yilmaz U. T. , Somer G.

TURKISH JOURNAL OF CHEMISTRY, cilt.35, ss.201-213, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 35 Konu: 2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3906/kim-1003-409
  • Dergi Adı: TURKISH JOURNAL OF CHEMISTRY
  • Sayfa Sayıları: ss.201-213

Özet

In this work, direct and indirect methods for determination of vitamin K-3 were developed using differential pulse polarography. The reaction between sulfite and the quinone (Q) form of vitamin K-3 was used for indirect determination, since the sulfite peak at -0.7 V is sharp and very reproducible in 0.1 M HAc NaAc (pH = 5.5). It was found that at pH 4.5-5.5, this reaction was quantitative and very fast when the temperature was 45 degrees C. For its direct determination, on the other hand, vitamin K-3 was standardized by the indirect method using standard sulfite as the reducing agent. The calibration graph for vitamin K-3 (in Q form), using the peak at -1.0 V in a HAc-NaAc medium with a pH of 5.5, was linear for concentrations ranging from 5 x 10(-7) to 3 x 10(-5) M, and the limit of detection (LOD) was 1.5 x 10(-7) M. The proposed methods were successfully applied to the determination of vitamin K-3 in a clinical injection solution and in blood serum.