Effects of sample volume on serum magnesium analyses in vacuum gel separator tubes


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Karaoğlan A., Yılmaz C., Gülbahar Ö., Paşaoğlu H.

TBD ULUSLARARASI BİYOKİMYA KONGRESİ 2022 33. ULUSAL BİYOKİMYA KONGRESİ, İzmir, Türkiye, 26 - 30 Ekim 2022, ss.2-3

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.2-3
  • Gazi Üniversitesi Adresli: Evet

Özet

OBJECTIVES: When serum samples are taken into vacuum gel tubes, the level of some analytes may change. In this study, in the blood taken in different brand vacuum gel tubes, the gel; It was investigated whether it had an effect on serum magnesium level. Materials and Methods: Small (1 ml) and large (5 ml) blood samples were collected randomly from 20 healthy volunteers in vacuum gel tubes of brands A and B. Simultaneously, another sample , was taken into a fifth tube with B brand gelless clot activator, left for 30 minutes, and centrifuged at 3500 rpm for 10 minutes. Magnesium levels were measured in clinical chemistry autoanalyzer with chemiluminescence method in triplicate. The mean of the results of the five tubes were compared with each other. RESULTS: Magnesium values of small and large amounts of serum taken into A brand tubes were found to be statistically significantly higher than the magnesium results of all B brand tubes (p<0.001). No significant difference was found as a result of the comparison of magnesium values of small and large amounts of serum taken into B brand tubes with each other and with the tubes without gel (p<0.05). Conclusions: In our study, it was observed that the high magnesium values detected in the gel tube of brand A were related to the separator gel, and the increase in magnesium levels became more pronounced as the sample volume decreased.