Various growth factors such as epidermal growth factor (EGF) are effective in wound healing. For this reason, it was targeted in our study to observe the impact of systematically given EGF on collagen and protein synthesis in hepatic tissue. Rats to which dorsolateral excisional wound was made were divided into 2 equal groups: 1-Untreated control group, 2-Systemic EGF applied group. A daily dose of EGF (10 ng/mL) was given to the animals intraperitoneally (ip) in the application group. Rats were sacrificed in 1st, 5th, 7th and 14th days of the study. Collagen and protein concentrations in hepatic tissues were measured spectrophotometrically. In consideration of the phases of systemic EGF application and its comparison with the controls, it was determined that EGF stimulates the synthesis of collagen up to 7 days and protein up to 5 days in liver and this stimulation stops in the later days and EGF begins to make pressure on collagen and protein synthesis.