Role of free radicals on mechanism of radiation nephropathy

Balabanli B., Turkozkan N., Akmansu M., Polat M.

MOLECULAR AND CELLULAR BIOCHEMISTRY, sa.1-2, ss.183-186, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1007/s11010-006-9240-5
  • Dergi Adı: MOLECULAR AND CELLULAR BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.183-186
  • Anahtar Kelimeler: kidney, irradiation, nitric oxide, Na/K-ATPase, vitamin A, TOTAL-BODY IRRADIATION, NITRIC-OXIDE, RETINOIC ACID, BETA-CAROTENE, L-ARGININE, NA,K-ATPASE, ANTIOXIDANT, KIDNEY, DAMAGE
  • Gazi Üniversitesi Adresli: Evet

Özet

Purpose: In our study, after applying a single dose of 612 cGy irradiation, we aimed to observe the role of free radicals on tissue damage in the kidney caused by radiation by measuring NO level, Na/K-ATPase activity and TBARS amount which is an indicator of free radical damage. On the other hand we investigated whether the tissue damage can be prevented by vitamin A or not. Materials and methods: This study was performed on three groups: 1. Control group 2. The group to which irradiation was administrated 3. The group which was given radiation + vitamin A. The irradiation group of animals were given a single dose of gamma irradiation at a sublethal dose. In the group which was administrated both irradiation + vitamin A, vitamin A was given for two days prior to irradiation. The amount of NO was measured by ESR spectroscopy, Na/K-ATPase and TBARS were measured by spectrophotometry. Results and conclusions: As a result of radiation mediated tissue damage in the kidney, we observed a NO loss, a decrease in Na/K-ATPase activitiy and an increase in TBARS amount. Although the administration of vitamin A before radiation, did not have any effect on NO loss and decrease in Na/K-ATPase.