Detection of p53 deletions using fish technique in the HL-60, Daudi, Raji and K-562 cell lines


Konac E. , ERGÜN M. A. , Sahin F., Ekmekci A.

Experimental Oncology, cilt.25, ss.33-35, 2003 (SCI Expanded İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 25 Konu: 1
  • Basım Tarihi: 2003
  • Dergi Adı: Experimental Oncology
  • Sayfa Sayıları: ss.33-35

Özet

The work is aimed on the analysis of chromosome 17 centromere and p53 gene-specific signals within interphase and metaphase, determination of p53 deletion ratios and the detection of the cell-cycle progress. The loss of heterozygosity (LOH) has been investigated using FISH method in 4 cell lines (HL-60, Daudi, Raji and K-562) and peripheral blood samples from 3 Burkitt's lymphoma patients and 2 healthy donors. It is concluded that FISH method may be used in aneuploidy as well as in detection of p53 deletions and mutations.