Regulation of periodontal ligament cell functions by interleukin-1 beta

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Agarwal S., Chandra C., Piesco N., Langkamp H., Bowen L., Baran C.

INFECTION AND IMMUNITY, vol.66, no.3, pp.932-937, 1998 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 66 Issue: 3
  • Publication Date: 1998
  • Doi Number: 10.1128/iai.66.3.932-937.1998
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.932-937
  • Gazi University Affiliated: No


Periodontal ligament (PDL) cells maintain the attachment of the tooth to alveolar bone. These cells reside at a site in which they are challenged frequently by bacterial products and proinflammatory cytokines, such as interleukin-1 beta (IL-1 beta), during infections. In our initial studies we observed that IL-1 beta down-regulates the osteoblast-like characteristics of PDL cells in vitro. Therefore, we examined the functional significance of the loss of the PDL cell's osteoblast-like characteristics during inflammation. an this report we show that, during inflammation, IL-1 beta can modulate the phenotypic characteristics of PDL cells to a more functionally significant lipopolysaccharide (LPS)-responsive phenotype. In a healthy periodontium PDL cells exhibit an osteoblast-like phenotype and are unresponsive to gram-negative bacterial LPS. Treatment of PDL cells with IL-1 beta inhibits the expression of their osteoblast-like characteristics, as assessed by the failure to express transforming growth factor beta 1 (TGF-beta 1) and proteins associated with mineralization, such as alkaline phosphatase and osteocalcin. As a consequence of this IL-1 beta-induced phenotypic change, PDL cells become responsive to LPS and synthesize proinflammatory cytokines. The IL-1 beta-induced phenotypic changes in PDL cells were transient, as removal of IL-1 beta from PDL cell cultures resulted in reacquisition of their osteoblast-like characteristics and lack of LPS responsiveness. The IL-1 beta-induced phenotypic changes occurred at concentrations that art frequently observed in tissue exudates during periodontal inflammation (0.05 to 5 ng/ml). The results suggest that, during inflammation in vivo, IL-1 beta may modulate PDL cell functions, allowing PDL cells to participate directly in the disease process by assuming LPS responsiveness at the expense of their normal structural properties and functions.