Determination of the origin of the marker chromosomes is very difficult and requires effort. Evaluation of the marker chromosomes of the pregnancy lost tissue could be the most difficult one as the contamination with maternal decidual cells complicates this process. Conventional cytogenetic techniques and fluorescence in situ hybridization technique or more advanced molecular techniques are preferred to determine the origin of these chromosomes. In this report, the study for identification of the origin of an abortion material derived from the primary tissue cell culture, with a 47,XX,+mar(15) karyotype was presented, by using an effective algorithmic approach which ended with genotyping. The result of the genotyping was informative, since the assumed abortion material actually belonged to the mother's decidual tissue. This brief study reminds the efficient algorithmic approaches to the determination process of the marker chromosomes of the abortion material.