High-fructose in drinking water initiates activation of inflammatory cytokines and testicular degeneration in rat


Yildirim O. G., Sumlu E., Aslan E., KOCA H. B., PEKTAŞ M. B., SADİ G., ...Daha Fazla

TOXICOLOGY MECHANISMS AND METHODS, cilt.29, sa.3, ss.224-232, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1080/15376516.2018.1543745
  • Dergi Adı: TOXICOLOGY MECHANISMS AND METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.224-232
  • Anahtar Kelimeler: Dietary fructose, inflammatory cytokines, testicular degeneration, testosterone, IGF-1R, GROWTH-FACTOR-I, INSULIN-RESISTANCE, DYSFUNCTION, OBESITY, PROLIFERATION, RESVERATROL, EXPRESSION, INJURY, KIT, MEN
  • Gazi Üniversitesi Adresli: Evet

Özet

The increased consumption of high-fructose in diet may contribute to high prevalence of metabolic syndrome in the world. The influence of high-fructose diet on male reproductive system has been poorly documented. In this study, we investigated the effects of dietary high-fructose on the expression of inflammatory cytokines in association with certain testicular proteins and sex hormones in the testis of rats. Fructose was given to the rats as 20% solution (7.8mg/kg) in drinking water for 15 weeks. Dietary high-fructose caused testicular degeneration, also decreased testicular concentration of testosterone and right testis absolute weight. This dietary intervention increased iNOS and TNF-alpha mRNAs as well as iNOS, NF-kappa B, and p-NF-kappa beta proteins, but decreased IL-10 and IL-6 mRNAs expressions, in testicular samples of rats. Moreover, testicular TNF-alpha, IL-1 beta, and iNOS and plasma IL-1 beta levels were significantly increased in rats fed with fructose. A marked increase in the expression level of IGF-1R protein was considered in testicular tissue of fructose-treated rats. The expression intensities of c-kit, claudin-1, and pan-cadherin were comparable in seminiferous tubules of control and fructose-treated rats. In conclusion, high-fructose intake of rats leads to activation of inflammatory cytokines, which is accompanied by testicular degeneration. These changes could be responsible for hormonal dysfunction with low intra-testicular testosterone level, which could be relevant to male infertility.