High-fructose in drinking water initiates activation of inflammatory cytokines and testicular degeneration in rat

Yildirim O. G. , Sumlu E., Aslan E., KOCA H. B. , PEKTAŞ M. B. , SADİ G., ...More

TOXICOLOGY MECHANISMS AND METHODS, vol.29, no.3, pp.224-232, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 3
  • Publication Date: 2019
  • Doi Number: 10.1080/15376516.2018.1543745
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.224-232
  • Keywords: Dietary fructose, inflammatory cytokines, testicular degeneration, testosterone, IGF-1R, GROWTH-FACTOR-I, INSULIN-RESISTANCE, DYSFUNCTION, OBESITY, PROLIFERATION, RESVERATROL, EXPRESSION, INJURY, KIT, MEN
  • Gazi University Affiliated: Yes


The increased consumption of high-fructose in diet may contribute to high prevalence of metabolic syndrome in the world. The influence of high-fructose diet on male reproductive system has been poorly documented. In this study, we investigated the effects of dietary high-fructose on the expression of inflammatory cytokines in association with certain testicular proteins and sex hormones in the testis of rats. Fructose was given to the rats as 20% solution (7.8mg/kg) in drinking water for 15 weeks. Dietary high-fructose caused testicular degeneration, also decreased testicular concentration of testosterone and right testis absolute weight. This dietary intervention increased iNOS and TNF-alpha mRNAs as well as iNOS, NF-kappa B, and p-NF-kappa beta proteins, but decreased IL-10 and IL-6 mRNAs expressions, in testicular samples of rats. Moreover, testicular TNF-alpha, IL-1 beta, and iNOS and plasma IL-1 beta levels were significantly increased in rats fed with fructose. A marked increase in the expression level of IGF-1R protein was considered in testicular tissue of fructose-treated rats. The expression intensities of c-kit, claudin-1, and pan-cadherin were comparable in seminiferous tubules of control and fructose-treated rats. In conclusion, high-fructose intake of rats leads to activation of inflammatory cytokines, which is accompanied by testicular degeneration. These changes could be responsible for hormonal dysfunction with low intra-testicular testosterone level, which could be relevant to male infertility.