Biomonitoring cytogenetic and oxidative-stress related damage in gas station attendants: Buccal micronucleus cytome assay and serum 8-OHdG levels

ELKAMA A., İlik N., Şentürk K., KARAHALİL B.

Archives of Environmental and Occupational Health, cilt.80, sa.7-8, ss.165-173, 2025 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 80 Sayı: 7-8
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/19338244.2025.2520850
  • Dergi Adı: Archives of Environmental and Occupational Health
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.165-173
  • Anahtar Kelimeler: Benzene, DNA damage, gasoline, occupational hazards, oxidative stress
  • Gazi Üniversitesi Adresli: Evet

Özet

This study assessed the genotoxicity and oxidative DNA damage associated with occupational exposure among gas station attendants. We performed buccal micronucleus cytome analysis and measured serum 8-OHdG levels in 32 pumpers and 32 control subjects. Cytotoxicity biomarkers were higher in the exposed group. No significant effect of smoking on cytogenetic parameters was found. In contrast, a higher frequency of genotoxicity and cytotoxicity biomarkers was observed in pumpers with more than 10 years of employment. Serum 8-OHdG levels were higher in pumpers. While the duration of working increased the serum 8-OHdG levels, smoking had no significant effect. Our findings reveal that gas station attendants are continuously exposed to benzene-induced toxicity at the cellular level. Workers may be at risk of developing cancer in the future, so they should be carefully monitored for the long-term effects.