Milling Effect on The Structural and Antibacterial Properties of ZnO and CuO Nanoparticles

Çinar Avar E., Loğoğlu E.

PHARMACEUTICAL CHEMISTRY JOURNAL, vol.56, no.7, pp.899-905, 2022 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 56 Issue: 7
  • Publication Date: 2022
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE
  • Page Numbers: pp.899-905
  • Gazi University Affiliated: Yes


In this work, ZnO and CuO nanocrystalline powders were prepared by method of high-energy ball milling. The milling process was performed at 450 rpm speed for 5, 10, 15, and 20 h periods of time. Structural and morphological properties of the powders were investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. Results confirmed the formation of single-phase nanocrystalline structure from micron-scale (as-received) material. The samples were tested for antibacterial activity against Gram-positive bacteria Bacillus cereus B9 (ATCC 25923), Bacillus licheniformis M30, Bacillus subtilis B1, Micrococcus luteus M3, Staphylococcus aureus (ATCC 6538) and Gram-negative bacteria Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa P7. The antibacterial activity was determined by the disc-diffusion method.