Akademik Veri Yönetim Sistemi
Turkiye Klinikleri J Med Sci, cilt.39, sa.2, ss.194-201, 2019 (Hakemli Dergi)
Objective:
Hypoxic preconditioning allows cells to gain resistance to hypoxic damage.
There are a
limited number of studies suggesting that hypoxic preconditioning increases
antioxidant capacity in the lung. In this study, we aimed to evaluate effects
of hypoxic preconditioning on oxidant/antioxidant systems in rat lung. Material and Methods: Rats were divided
into 4 groups: control, preconditioning (PC) (10% O2), severe hypoxia (SH) (7%
O2) and PC + SH. The parameters related to oxidative stress and antioxidant
defense mechanisms, which are malondialdehyde (MDA)levels, total oxidant system
(TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), superoxide
dismutase (SOD) and glutathione (GSH) activity, were measured. Results were
evaluated using the One-Way ANOVA and t-test; p<0.05 was considered
significant. Results: Compared with the
control group, MDA levels decreased in all hypoxic groups; decrements in PC and
SH groupswere statistically significant. Compared with the control group,
levels of TOS showed a significant increase in the PC+SH group. OSI of PC+SH
group was significantly higher than other groups. There was no significant
difference in TAC levels between the groups. Compared to other groups, SOD
activity showed a significant decrease in the PC+SH group. GSH levels showed a
significant decrease in PC+SH group compared to both control and PC group.
Conclusion: Our findings suggest that hypoxic preconditioning does not have an
effect on antioxidant defense systems in lungs, but severe hypoxia does affect
oxidant/antioxidant systems.
Keywords: Hypoxia; hypoxic
preconditioning; lung; reactive oxygen species; oxidant/antioxidant systems