Akademik Veri Yönetim Sistemi
Agribalkan 2022, IV. Balkan Agricultural Congress, Edirne, Türkiye, 31 Ağustos - 02 Eylül 2022, ss.332
Beta glucan (β-glucan), is very potent immunomodulators with effects on immune, sourced
from cereals, fungi and yeasts. Based on the some studies have reported that β-glucans have
various bioactivities such as antioxidant, anti-inflammatory, antiviral and antiproliferatif
properties. Due to these positive effects on health, beta glucans have been used as a dietary
supplement in many countries. The aim of this study, the potential cytotoxic/antiproliferatif
effect of β-glucan has been evaluated in human hepatocellular carcinoma HepG2 cells using
Mtt and Neutral red uptake (Nru) assays. These assays are widely used popular biomarker
detected the cytotoxicity. β-glucans were isolated from the yeast strains Pichia kudriavzeii
M13 and Kluyveromyces marxianus M59. HepG2 cells were grown to confluence at 37°C under
5% CO2 in flasks with Dulbecco’s Modified Eagle Serum (DMEM) including 10% fetal
bovine serum (FBS), 1 (%) penicillin/streptomycin and 2 mM L-glutamine. Cells were treated
with different concentrations of β-glucans (7.5 – 480 µg/mL) at 24 hour treatment period. A
negative control (distilled water) was also maintained. As a result, β-glucan obtained from
Pichia kudriavzeii M13 strain did not significant affect on the cell viability using both Mtt and
Nru assays. However, β-glucan isolated from Kluyveromyces marxianus M59 strain was
significantly decreased the cell viability at 60-480 µg/mL using Mtt assay. Additionally, the
half of inhibitory (IC50) value was determined as 480 µg/mL for 24 hour using Mtt assay.
Consequently, it has been determined that β-glucan isolated from Kluyveromyces marxianus
M59 strain has a cytotoxic activity especially at higher concentrations on HepG2 cells.