Immobilization of beta-galactosidase on Novel Polymers Having Schiff Bases

Aynaci E., SARI N., TÜMTÜRK H.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.39, sa.4, ss.259-266, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 4
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3109/10731199.2011.555837
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.259-266
  • Anahtar Kelimeler: Polymeric support, Storage capacity, Immobilization, Lineweaver-Burk plot, beta-galactosidase, Schiff bases, INVERTASE, STABILIZATION, SUPPORTS, GALACTOOLIGOSACCHARIDES, PURIFICATION, ADSORPTION, ENZYMES
  • Gazi Üniversitesi Adresli: Evet

Özet

We have developed a strategy to immobilize beta-galactosidase as a model enzyme by using polymeric supports having Schiff bases, which were prepared from (aminomethyl) polystyrene and 2-phenlyindole-3-carboxaldehyde by condensation. beta-galactosidase was immobilized onto the new polymer supports via covalent bonds. The influence of temperature, pH, reusability, and storage capacity on the free and immobilized beta-galactosidase was investigated. Our results indicate that the (aminomethyl) polystyrene with Schiff bases is most suitable for the immobilization of beta-galactosidase. These kinds of new supports can be used for the immobilization of beta-galactosidase due to their strong storage capacity and reusability.