Akademik Veri Yönetim Sistemi
9th International Academic Studies Conference (UBCAK 2022), Ankara, Türkiye, 03 Ağustos 2022, ss.109-110
Magnetic resonance imaging (MRI) is a
consolidated imaging technique for the multiparametric evaluation of tissues in
various diseases. Despite the high resolution capability, in almost half of all
the MRI examinations, the image contrast is insufficient, resulting in the use
of contrast agents. Gadolinium-based contrast agents (GBCAs) are widely used to
increase the accuracy of the diagnosis, one of the most preferred among them is
gadobutrol, is administered for MRI of the central nervous system, kidneys,
liver, and contrast increment in magnetic resonance angiography. Its use has
also raised concerns about whether it has adverse effects on people's health.
Therefore, in this study, the genotoxic effects of gadobutrol were assessed by
Micronucleus Test in human peripheral lymphocytes in vitro. For this purpose, depending on both the LD50 value of gadobutrol in rodents and the
recommended dose of use in humans, five concentrations (7,000, 14,000, 28,000,
56,000, and 112,000 μg/mL) of gadobutrol were applied to lymphocytes obtained
from three healthy donors. A negative (distilled water) and a positive
(Mitomycin C, 0.20 μg/mL) control were also used. In analyzes performed
by examining binuclear cells, it was observed that gadobutrol forms binucleates
with 1 micronucleus in human lymphocytes. It was determined that this contrast
agent caused increases in MN frequency compared to the negative control, but
these increases were not at a significant level. On the other hand,
gadobutrol caused a decrease in the Nuclear Division Index (NDI) compared to
the negative control, but these reductions were not statistically significant.
These results indicate that gadobutrol did not have strong clastogenic and/or aneugenic effects in the MN test
in human lymphocytes. However, further in vitro and in vivo genotoxicity tests should be
carried out to reduce health concerns.