Investigating the in-vitro diuretic activity and rosmarinic acid content of extracts from Olea europaea L. Ayvalik and Olea europaea L. Gemlik for potential health and medicinal purposes.

International Conference On Nanobiotechnology and Pharmacy, İstanbul, Türkiye, 9 - 10 Temmuz 2024, ss.23

Yayın Türü: Bildiri / Özet Bildiri
Basıldığı Şehir: İstanbul
Basıldığı Ülke: Türkiye
Sayfa Sayıları: ss.23
Gazi Üniversitesi Adresli: Evet

Özet

The Olea europaea L., a member of the Oleaceae family, has long been utilized in traditional medicine across Mediterranean regions [1]. Olive leaf and extracts are popular in complementary and alternative medicine for their potential to reduce blood pressure in animals [2] and to act as an antipyretic and diuretic for malaria in humans [3-5].

The aim of this study is to determine diuretic activity and quantity of rosmarinic acid in leaf extracts of Olea europaea L. Ayvalik, Olea europaea L. Gemlik, and a mixture of two taxa’s leaves (50:50, w/w) in Turkey. All Olea europaea L. taxa were extracted from their leaves using the infusion method with hot water for two hours. According to results of UPLC-UV analysis, the infusion extraction of a mixture of leaves with the highest quantity of rosmarinic acid. In the in-vitro enzymatic diuretic analysis, the infusion extraction of a mixture of leaves showed the highest inhibition of carbonic anhydrase enzyme with 9.39 µg/mL (equivalent to acetazolamide) (Table 1).

Table 1. Results of the diuretic activity experiments

Olea europeae Species	Concentration (equivalent acetazolamide) (µg/mL)	Inhibition with dilution factor
Ayvalık	1.89	59.38
Gemlik	1.94	67.59
Mixture of species	9.39	317.23

In light of these results, it was noted that the infusion extraction of a mixture of leaves exhibited the most significant diuretic activity, which effect may be associated with the level of rosmarinic acid and inhibition of carbonic anhydrase.