Investigating the in-vitro diuretic activity and rosmarinic acid content of extracts from Olea europaea L. Ayvalik and Olea europaea L. Gemlik for potential health and medicinal purposes.

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Büker E., Casoni D.

International Conference On Nanobiotechnology and Pharmacy, İstanbul, Türkiye, 9 - 10 Temmuz 2024, ss.23, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.23
  • Gazi Üniversitesi Adresli: Evet

Özet

The Olea europaea L., a member of the Oleaceae family, has long been utilized in traditional medicine across Mediterranean regions [1]. Olive leaf and extracts are popular in complementary and alternative medicine for their potential to reduce blood pressure in animals [2] and to act as an antipyretic and diuretic for malaria in humans [3-5].

The aim of this study is to determine diuretic activity and quantity of rosmarinic acid in leaf extracts of Olea europaea L. Ayvalik, Olea europaea L. Gemlik, and a mixture of two taxa’s leaves (50:50, w/w) in Turkey.  All Olea europaea L. taxa were extracted from their leaves using the infusion method with hot water for two hours. According to results of  UPLC-UV analysis, the infusion extraction of a mixture of leaves with the highest quantity of rosmarinic acid. In the in-vitro enzymatic diuretic analysis, the infusion extraction of a mixture of leaves showed the highest inhibition of carbonic anhydrase enzyme with 9.39 µg/mL (equivalent to acetazolamide) (Table 1).

Table 1. Results of the diuretic activity experiments

Olea europeae Species

Concentration (equivalent acetazolamide) (µg/mL)

Inhibition with dilution factor

Ayvalık

1.89

59.38

Gemlik

1.94

67.59

Mixture of species

9.39

317.23

 

In light of these results, it was noted that the infusion extraction of a mixture of leaves exhibited the most significant diuretic activity, which effect may be associated with the level of rosmarinic acid and inhibition of carbonic anhydrase.