Effects of L-arginine on the release of heme oxygenase-1 on ischemia-reperfusion induced acute pancreatitis in rats

DİKMEN K., BOSTANCI H., Gobut H., Yavuz A., GÜLBAHAR Ö., AKYÜREK N., ...Daha Fazla

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, cilt.11, sa.10, ss.11061-11069, 2018 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 10
  • Basım Tarihi: 2018
  • Dergi Adı: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.11061-11069
  • Anahtar Kelimeler: Ischemia/reperfusion induced pancreatitis, L-arginine, heme-oxygenase, NITRIC-OXIDE, MICROCIRCULATORY DYSFUNCTION, CARBON-MONOXIDE, ISCHEMIA/REPERFUSION, LIPOPOLYSACCHARIDE, EXPRESSION, SEVERITY, ANTIOXIDANT, MODULATION, INDUCTION
  • Gazi Üniversitesi Adresli: Evet

Özet

Background: Ischemia-reperfusion (I/R) is a causative factor in the pathogenesis of acute pancreatitis. L-arginine plays a key role in the relationship between microcirculatory disorders and I/R injuries. Heme oxygenase- 1 (HO-1) has been identified as a stress protein induced in many cell types by various stimulants, such as oxidative stress. Aim: The present study aimed to investigate the effects of L-arginine on HO-1 in pancreatitis resulting from ischemia and reperfusion. Materials and Methods: Pancreatic arterial vessels were prepared and clamped for 1 hour, then released after 3 hours. Animals were divided into 5 groups, sham, L-arginine+ischemia without reperfusion, saline+ischemia without reperfusion, L-arginine+ischemia with reperfusion, and saline+ischemia with reperfusion. Blood was collected for amylase and myeloperoxidase (MPO) and pancreatic tissue was collected for superoxide dismutase (SOD), malondialdehyde (MDA), heme-oxygenase-1 (HO-1), and histopathologic grading of pancreatic injuries. Results: Levels of amylase, MPO, SOD, and MDA in the L-arginine+ischemia without reperfusion and L-arginine+I/R groups were lower than in saline+ischemia without reperfusion and saline+I/R groups (p<0.05). In the L-arginine+I/R group, these parameters were lower than the L-arginine+ischemia without reperfusion group (p<0.05). In the saline+ischemia without reperfusion group, MPO, SOD, and MDA levels were significantly higher, compared to the saline+I/R group (p< 0.05). HO-1 expression was significantly higher in the L-arginine treated groups. It was highest in the L-arginine+I/R group (p< 0.05). Histopathological findings also supported the protective roles of L-arginine. Conclusion: Present data suggests that L-arginine, inducing HO-1 expression, could be useful in preventing oxidative damage associated with I/R induced pancreatitis.