Akademik Veri Yönetim Sistemi
The 16th International Symposium on Biological and Environmental Reference Materials (BERM 16), Halifax, Kanada, 1 - 04 Haziran 2025, (Tam Metin Bildiri)
The National Institute of Standards and Technology (NIST) and the National Institutes of Health, Office of Dietary Supplements (NIH-ODS), are collaborating to advance non-targeted analytical (NTA) methods for characterizing botanical and other natural product dietary supplement ingredients. While traditional approaches focus on quantifying specific marker compounds, NTA has the potential to capture broader compositional differences that may aid in authentication and quality assessment.
This study investigated the use of LC-QTOF-MS and NMR spectroscopy to authenticate Panax quinquefolius (American ginseng) by evaluating differences across a representative set of samples. The authenticated sample set included 51 American ginseng root samples and 4 leaf samples, 41 P. ginseng (Korean or Asian ginseng) root samples and 20 leaf samples, and 1 P. notoginseng (Chinese or tienchi ginseng) root sample. Additional American and Korean ginseng root and leaf samples, as well as Chinese ginseng root samples, were obtained from commercial sources.
Unless received as a powder, samples were cryo-ground to a fine powder. Extracts were prepared using 70% methanol for LC-QTOF-MS and methanol-d₄ for NMR. Alignment and feature extraction of LC-QTOF-MS data were investigated using MZMine and Progenesis QI. Extracted MS features (ions, areas, retention times) and NMR spectral data were assessed using PCA followed by evaluation with several multivariate classification methods. The results of these investigations will be presented.
Ongoing work focuses on assessing the complementarity of these two analytical techniques in distinguishing American ginseng from related species and selecting representative materials for an interlaboratory study. The long-term goal is to evaluate method reproducibility and robustness across different analytical platforms, contributing to improved strategies for authenticating botanical ingredients in dietary supplements.