Anti-Acetylcholinesterase and Antioxidant Appraisal of the Bulb Extracts of Five Sternbergia Species

ERDOĞAN ORHAN İ. , Yilmaz B. S. , ALTUN M. L. , Saltan G., Sener B.

RECORDS OF NATURAL PRODUCTS, cilt.5, sa.3, ss.193-201, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 5 Konu: 3
  • Basım Tarihi: 2011
  • Sayfa Sayıları: ss.193-201


In the current study, we examined anti-acetylcholinesterase (AChE) and antioxidant activities of the ethyl acetate, methanol, and water extracts from the bulbs of Turkish Sternbergia Waldst. & Kit. (Amaryllidaceae) species; S. candida, S. clusiana, S. fisheriana, S. lutea subsp. lutea, and S. lutea subsp. sicula. Anti-AChE activity was tested by spectrophotometric method of Ellman using ELISA microplate reader at 50, 100, and 200 mu g mL(-1) concentrations. Antioxidant activity of the extracts was evaluated by DPPH radical scavenging activity, ferrous ion-chelating capacity, ferric-reducing antioxidant power, and beta-carotene bleaching assays at 500, 1000, and 2000 mu g mL(-1). Total phenol and flavonoid contents of the extracts were determined via Folin-Ciocalteau's and AlCl(3) reagents, respectively. The ethyl acetate extract of S. fischeriana was the most active in anti-AChE assay (90.94% and 98.02% of inhibitions at 100 and 200 mu g mL(-1) concentrations, respectively. Antioxidant activity of the extracts was found to be not significant.