Simultaneous determination of carbamazepine and its active metabolite carbamazepine-10, 11-epoxide in human plasma by UPLC

Ates Z., Oezden T., Oezilhan S., Toptan S.

CHROMATOGRAPHIA, cilt.66, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1365/s10337-007-0299-2
  • Dergi Adı: CHROMATOGRAPHIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Gazi Üniversitesi Adresli: Hayır

Özet

A simple method for the determination of carbamazepine and its active metabolite carbamazepine- 10, 11 -epoxide by ultra performance liquid chromatography (UPLC) with ultraviolet absorbance detection (TUV) was developed. The method involves a two-step protein precipitation by liquid-liquid extraction. Phenytoin sodium was used as the internal standard. The separation was carried out on Acquity C18 column with acetonitrile: methanol: KH2PO4 buffer (adjusting pH to 4.6 with 85% o-phosphoric acid) (180/180/170, v/v/v) as the mobile phase at a flow rate of 0.4 mL min(-1). Linear detection response was obtained for concentrations ranging from 50 to 5,000 ng mL(-1). The limit of quantification (LOQ) was 50 ng mL(-1). The method was validated successfully for the determination of carbamazepine and its active metabolite carbamazepine- 10, 11 -epoxide, which can be applied through pharmacokinetics and bioequivalence studies.