In Planta Evidence for the Involvement of a Ubiquitin Conjugating Enzyme (UBC E2 clade) in Negative Regulation of Disease Resistance

Unver T., Turktas M., Budak H.

PLANT MOLECULAR BIOLOGY REPORTER, cilt.31, sa.2, ss.323-334, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1007/s11105-012-0502-y
  • Dergi Adı: PLANT MOLECULAR BIOLOGY REPORTER
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.323-334
  • Anahtar Kelimeler: Ubiquitylation, AtUbc2, VIGS, Plant disease resistance, SGT1, F-BOX PROTEIN, FUNCTIONAL-CHARACTERIZATION, MILDEW RESISTANCE, 26S PROTEASOME, CELL-DEATH, ARABIDOPSIS, DEFENSE, SGT1, JASMONATE, EXPRESSION
  • Gazi Üniversitesi Adresli: Hayır

Özet

One of the three main components of the ubiquitylation system is an ubiquitin-conjugating enzyme (UBC, E2) that attaches ubiquitin (Ub) to a substrate. A strong link has been discovered between ubiquitylation and regulation of plant defense responses against plant pathogens. In this study, the role of UBC in response to pathogen attack is investigated in Arabidopsis thaliana as it has three Ubc gene homologs (Ubc1-1, Ubc2-1, and Ubc3-1). Analysis of single mutants of these three Ubc genes revealed enhanced disease resistance. Moreover, virus-induced gene silencing (VIGS) analysis revealed an enhanced level of resistance of Nicotiana benthamiana following inoculation with avirulent Pto DC3000 and virulent Pto DC3000 hopQ1-1 strains. In addition, RAR1, SGT1, and HSP90 mRNA levels are up-regulated following Ubc2 silencing. This study shows that UBC2 negatively regulates disease resistance via SGT1 level. Sub-cellular localization assay of UBC2 protein indicates its nuclear and cytoplasmic localization in planta.