Eleven species of the Boraginaceae family collected in Turkey were analyzed with respect to the amount of gamma-linolenic acid as well as fatty acid composition. gamma-Linolenic acid was found in all species, except for Heliotropium lasiocarpum Fisch. & Mey. and Heliotropium dolosum De Not. Seed oil content ranged from 1.5% in H. lasiocarpum to 22.0% in Anchusa leptophylla ssp. leptophylla. The content of gamma-linolenic acid ranged from 1.7% of total fatty acids in Paracaryum racemosum (Schreber) Britten var. racemosum to 13.7% in A. leptophylla Roemer & Shultes ssp. leptophylla. According to data from our study, A. leptophylla ssp. leptophylla (13.7%), Anchusa azurea Miller var. azurea (11.9%) and Echium plantagineum L. (12.2%) might be considered as new sources of gamma-linolenic acid.