Protective Effect of Ozone on Liver Ischemia–Reperfusion (I/R) Injury in Streptozotocin-Induced Diabetic Rats
Bratislava Medical Journal, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s44411-026-00803-y
- Dergi Adı: Bratislava Medical Journal
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Ultimate (EBSCO)
- Anahtar Kelimeler: Diabetes mellitus, Ischemia–reperfusion, Liver, Ozone
- Gazi Üniversitesi Adresli: Evet
Özet
Objective: The aim of this study was to investigate the effects of ozone therapy on the liver, one of the organs most affected by ischemia–reperfusion damage in people with diabetes. Materials and methods: Thirty male Wistar albino rats were used in this study. The rats were randomly divided into four groups: the control group (C) without diabetes (n = 6), the diabetes control (DC) group (n = 8), the diabetes ischemia–reperfusion (DIR) group (n = 8), and the diabetes ischemia–reperfusion-ozone (DIR-O) group (n = 8). Twenty-four of the rats were given 55 mg/kg IP streptozotocin to induce diabetes. The experiment lasted one month to evaluate the effects of diabetes on the organs. At the end of one month, an I/R injury was induced in the liver of the DIR and DIR-O groups. The DIR-O group was given an intraperitoneal ozone–oxygen mixture of 0.7 mg/kg (50 µg/mL) one hour before I/R injury was induced. All groups were then sacrificed under deep anesthesia by draining blood from the intra-abdominal aorta, and liver tissue was removed and sent for histopathological and biochemical examination. The tissue samples were stored in a 10% formaldehyde solution until histopathological evaluation. The samples for biochemical evaluation were stored at − 70 °C. Results: The histopathological examination of the cells revealed significant differences among the groups in terms of hydropic degeneration, sinusoidal dilatation, necrosis, and mononuclear cell infiltration in the parenchyma. These findings were significantly higher in the DIR group than in the C and DC groups, and lower in the DIR-O group. Conclusion: Our study showed that ozone therapy can significantly reduce the destructive effects of diabetes and I/R damage on the liver. Further studies are needed to determine the most effective dose and method of ozone therapy for clinical application.