Effects of triterpene saponins from Astragalus species on in vitro cytokine release


Yesilada E., Bedir E., Calis I., Takaishi Y., Ohmoto Y.

JOURNAL OF ETHNOPHARMACOLOGY, vol.96, pp.71-77, 2005 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 96
  • Publication Date: 2005
  • Doi Number: 10.1016/j.jep.2004.08.036
  • Title of Journal : JOURNAL OF ETHNOPHARMACOLOGY
  • Page Numbers: pp.71-77
  • Keywords: Astragalus spp., Fabaceae, cycloartane triterpene glycosides, interleukin-2, interleukin-4, interleukin-1 beta, interleukin-8, interferone-gamma, oleanan triterpene glycosides, triterpene saponins, tumor necrosis factor-alpha, CYCLOARTANE-TYPE GLYCOSIDE, BUTYL-N'-BUTANOLNITROSOAMINE, INFLAMMATORY CYTOKINES, INTERLEUKIN-2, THERAPY, ROOTS, MICROCEPHALUS, CARCINOGEN, TOXICITY, TROJANUS

Abstract

Roots of Astragalus species are used to treat leukemia and for wound healing in Turkish folk medicine. In order to evaluate this information, the effect of 13 cycloartane- and 1 oleanan-type triterpene saponins isolated from Turkish species (Astragalus brachypterus, Astragalus cephalotes, Astragalus microcephalus, and Astragalus trojanus), as well as methanol extracts from the roots of three Astragalus species (Astragalus cephalotes, Astragalus oleifolius and Astragalus trojanus) were studied. Cytokine concentrations of interleukins IL-1beta, IL-8 and TNF-alpha after bacterial lipopolysaccharide (LPS) and IL-2, IL-4 and INF-gamma after phorbolacetate (PHA) stimulation were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits.