Bovine serum albumin partitioning in an aqueous two-phase system: Effect of pH and sodium chloride concentration


Gunduz U. , Korkmaz K.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, cilt.743, ss.255-258, 2000 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 743
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1016/s0378-4347(99)00498-3
  • Dergi Adı: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
  • Sayfa Sayıları: ss.255-258

Özet

The partitioning of bovine serum albumin (BSA) in a polyethylene glycol 3350 (8% w/w)-dextran 37 500 (6% w/w)-0.05 M phosphate aqueous two-phase was investigated at different pHs, at varying concentrations of sodium chloride at 20 degrees C. The effect of NaCl concentration on the partition coefficient of BSA was studied for the PEG-dx systems with initial pH values of 4.2, 5.0, 7.0, 9.0, and 9.8. The NaCl concentrations in the phase systems with constant pH value were 0.06, 0.1, 0.2, 0.3, and 0.34 M. It was observed that the BSA partition coefficient decreased at concentrations smaller than 0.2 M NaCl and increased at concentrations greater than 0.2 M NaCl for all systems with initial pHs of 4.2, 5.0, 7.0, 9.0, and 9.8. It was also seen that the partition coefficient of BSA decreased as the pH of the aqueous two-phase systems increased at any NaCl salt concentration studied. (C) 2000 Elsevier Science B.V. All rights reserved.