Akademik Veri Yönetim Sistemi
Fabad Journal of Pharmaceutical Sciences, cilt.32, sa.1, ss.23-32, 2007 (Scopus)
Two spectroscopic methods (absorbance ratio and Vierordt) were compared with high performance liquid chromatography (HPLC) for quantitative determination in dissolution tests of hydrochlorothiazide (HCT) and spironolactone (SPL) in commercial tablets. A 260 nm wavelength was chosen as the isosbestic point in the absorbance ratio method, and absorbance ratios of A269/A260 nm for HCT and A242/A260 nm for SPL were used for calculation of regression equations. For the Vierordt method, A11 values (1%, 1 cm) calculated at 242 nm and 269 nm for both substances were used for quantitative analysis of HCT and SPL. In the HPLC method, simultaneous determination of HCT and SPL from dissolution medium was achieved using the mobile phase containing water-methanol-phosphate buffer (71:25:4 v/v/v), pH: 3.1±0.1, in a Luna 5 μm C18 (250 x 4.6 mm) reversed phase column. Mefruside was chosen as the internal standard and PDA detection was carried out at 286 nm (flow rate 0.7 m.L.min-1). Dissolution tests in commercial tablets were carried out according to USP XXVI paddle method in 0.1 N HCl containing 0.1% sodium lauryl sulfate at 75 rpm at 37 ± 0.5°C. Comparison of the dissolution data from the HPLC and two spectroscopic methods indicated that spectroscopic and HPLC methods showed good correlation. Therefore, it was concluded that both spectroscopic method as well as HPLC can be used for routine analysis of HCT and SPL in dissolution tests of commercial tablets.