Investigation of the effects of stobadine on the antioxidant enzymes in carbon tetrachloride mediated brain toxicity karbon tetraklorür aracılı beyin toksisitesinde antioksidan enzimler üzerine stobadinin etkisinin araştırılmas


Bayraktar N., Devay S. D., Taslipinar M. Y., Ucankus N. L., Omeroglu S., Gumuslu S., ...Daha Fazla

Turkish Journal of Biochemistry, cilt.36, sa.4, ss.283-289, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 4
  • Basım Tarihi: 2011
  • Dergi Adı: Turkish Journal of Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.283-289
  • Anahtar Kelimeler: Antioxidant enzymes, Brain and stobadine, Carbon tetrachloride
  • Gazi Üniversitesi Adresli: Evet

Özet

Objectives: The aim of the present study was to investigate the relation between brain damage induced by carbon tetrachloride and superoxide dismutase, catalase, glutathione pero-xidase, and glutathione S-transferase enzyme activities. Also, the other aim was to evaluate the effect of stobadine on this metabolism. We particularly choose brain tissue as an experimental model because it contains reach polyunsaturated fatty acids which is one of the most important targets of the free radicals. Another important reason for choosing brain as a model is the limited studies with brain tissue damaged with carbon tetrachloride that compared to numerous ones with hepatic tissue. Methods: Rats were randomly separated into four experimental groups as control (n=10), stobadine (n=10), carbon tetrachloride (n=10), carbon tetrachloride+ stobadine (n=10). Results: Carbon tetrachloride administration reduced superoxide dismutase, catalase, glu-tathione peroxidase, and glutathione S-transferase enzyme activities. Catalase, superoxide dismutase and glutathione S-transferase enzyme activities of the carbon tetrachloride+ stobadine group nearly approached the levels of the control group. On the contrary, glutathione peroxidase activity could not access the normal levels. Histopathologic results showed that carbon tetrachloride induced morphologic damage and edema in the brain cells. In spite of limited improvement in the carbon tetrachloride + stobadine group we found eosinophilia and edemas at the neurons and neuroglial cells, respectively. Conclusions: It could be suggested that glutathione peroxidase enzyme can be more important antioxidant enzyme to protect brain cells against the oxygen or carbon tetrachloride based free radicals. The authors do not have a conflict of interest.