Objective: Silene L. (Caryophyllaceae) species are traditionally used for the treatment of inflammation, urinary inflammation, eye trouble, skin problem, stomach ache, dysentery, tooth decay, fever, headache, malaria, pimples and backache. Chemical ingredients of Silene species consist of flavonoids, anthocyanidins, terpenoids, triterpene saponins, phytoecdysteroids, benzenoids, vitamins, and show antioxidant, anti- inflammatory, antitumor and antiviral activities. Silene ruscifolia (Hub.-Mor. & Reese) Hub.-Mor. is called "gizli nakıl" in Türkiye. Material and Method: The plant material was collected from Beynam Forest (Ankara/Türkiye). The aerial parts of the plant were extracted with methanol in an ultrasonic bath. HPLC system (Agilent 1260 Series) with an autosampler, binary pump, column oven, and a UV detector was coupled with an iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) with a Dual Spray Agilent Jet Stream electrospray ionization source. Agilent TC C-18 (4.6 mm x 150 mm x 5 μm) column was used for the separation of the compounds. The GC-MS analysis of extract was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used to determine and identify the compounds. Result and Discussion: LC-MS Q-TOF analysis showed that S. ruscifolia contained rutin, narcissin, luteolin, isorhamnetin, rhamnetin and quercetin dimethyl ether. GC-MS analysis showed that the extract had the highest content of sugar (50.5%) and sugar alcohols (46.39%). It also contains carboxylic acid (0.47%), fatty acid (0.64%), sugar acid (0.42%), glycoside (0.48%), carotenoids (0.61%) and benzoic acid ester (0.49%). D-pinitol has the highest content in the extract with 41.14%.