Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques

Sezik E., Aslan M., Yesilada E., Ito S.

LIFE SCIENCES, cilt.76, sa.11, ss.1223-1238, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 76 Sayı: 11
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.lfs.2004.07.024
  • Dergi Adı: LIFE SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1223-1238
  • Anahtar Kelimeler: C-glycosides, Gentiana olivieri Griseb., Gentianaceae, hypoglycaemic, isoorientin, streptozotocin-induced diabetes, glucose-hyperglycaemic rats, STREPTOZOTOCIN-DIABETIC RATS, TRADITIONAL MEDICINE, FOLK MEDICINE, PRUNUS-DAVIDIANA, TURKEY, MICE, PHOSPHODIESTERASE, HYPERGLYCEMIA, FLAVONOIDS, MELLITUS
  • Gazi Üniversitesi Adresli: Evet

Özet

Hypoglycemic effect of Gentiana olivieri Griseb. (Gentianaceae) flowering herbs on oral administration were studied using in vivo models in normal, glucose-hyperglycemic and streptozotocin-induced diabetic rats. Through in vivo bioassay-guided fractionation processes isoorientin, a known C-glycosylflavone, was isolated from the ethylacetate fraction by silica gel column chromatography as the main active ingredient from the plant. Isoorientin exhibited significant hypoglycemic and antihyperlipidemic effects at 15 mg/kg b.w.dose. Isoorientin concentration of the extracts and fractions were determined by HPLC in order to establish a correlation between the hypoglycaemic activity. (C) 2004 Elsevier Inc. All rights reserved.