Assessment of Enzyme Inhibitory and Antioxidant Activities of Lignans from Taxus baccata L.


Kucukboyaci N., ERDOĞAN ORHAN İ. , Sener B., Nawaz S. A. , Choudhary M. I.

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, cilt.65, ss.187-194, 2010 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 65
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1515/znc-2010-3-404
  • Dergi Adı: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Sayfa Sayıları: ss.187-194

Özet

Phytochemical investigations of Taxus baccata L. by successive chromatographic methods resulted in the isolation of the lignans lariciresinol (1), taxiresinol (2), 3'-demethylisolariciresinol-9'-hydroxyisopropylether (3), isolariciresinol (4), and 3-demethylisolariciresinol (5) as well as taxoids. Compounds 1-5 were evaluated for their acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and lipoxygenase (LOX) inhibitory activities, which play a role in the pathogenesis of Alzheimer's disease (AD), by in vitro spectrophotometric methods, while they were also screened for their antioxidant capacity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferrous ion-chelating effect, and ferric-reducing antioxidant power (FRAP) at 125, 250, 500, and 1000 mu g ml(-1). All compounds exhibited a moderate inhibition against both BChE and LOX, whereas they were inactive towards AChE. The compounds displayed a great scavenging activity against DPPH especially at 500 and 1000 mu g ml(-1). Besides, they were found to exert noteworthy reducing antioxidant power on ferric ions. In particular, the FRAP of compounds 2 (3.552 +/- 0.02), 4(3.021 +/- 0.71), and 5 (3.533 +/- 0.01) were as high as that of the reference chlorogenic acid (3.618 +/- 0.01) at 1000 mu g ml(-1). None of the compounds exhibited chelating ability against ferrous ions.