DETERMINATION OF SOME VIRULENCE FACTORS IN STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS AND ENTEROCOCCUS FAECIUM ISOLATED FROM MEAT AND MILK PRODUCTS


Gündoğan N., Ataol O., Torlak F. O.

JOURNAL OF FOOD SAFETY, vol.33, no.4, pp.387-393, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1111/jfs.12062
  • Journal Name: JOURNAL OF FOOD SAFETY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.387-393
  • Gazi University Affiliated: Yes

Abstract

The aim of this work is to characterize Staphylococcus aureus, Enterococcusfaecalis and Enterococcusfaecium isolated from meat and milk products in Ankara, Turkey. A total of 40 S.aureus, 55 E.faecalis and 30 E.faecium isolates were isolated from raw calf meat (minced), chicken drumsticks, raw milk, ice cream and white cheese samples. A high proportion of S.aureus isolates had slime formation (80%), biofilm formation (70%), deoxyribonuclease (DNase) activity (53%), hemolytic activity (40%), protease production (80%) and lipase production (45%). The frequency of positive slime formation, DNase activity, hemolysis and protease production for E.faecalis isolates was 36, 5, 7 and 16%, and for E.faecium isolates was 50, 10, 13 and 6%. Neither biofilm formation nor lipase production was detected in Enterococcus isolates. It was found that S.aureus isolates were resistant to penicillin (77%), ampicillin (67%), erythromycin (30%), tetracycline (25%), chloramphenicol (20%) and gentamicin (17%), but they were sensitive to vancomycin and teicoplanin. All Enterococcus spp. isolates were sensitive to ampicillin, chloramphenicol, teicoplanin, tetracycline and vancomycin, but they were resistant to erythromycin (47%), gentamicin (14%) and penicillin (4%).