Standardization of Infusion of<i> Lycium</i><i> barbarum</i> Grown by Organic Farming Methods and Enzyme Inhibitory and Antioxidant Activities


Ozupek B., PEKACAR S., Orhan D. D.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.27, sa.1, ss.26-37, 2024 (ESCI) identifier identifier

Özet

The cultivation of medicinal and aromatic plants with conventional and organic farming techniques in order to protect biodiversity due to depleted natural resources is becoming increasingly common. The expense of organic farming techniques necessitates more careful selection of the plants to be grown. Evaluation of the bioactivity and phytochemical contents of these plants is important for the pharmaceutical and food industry. In this study, the antioxidant, antidiabetic, anticholesterolemic and antiobesity activities of the extracts obtained from the fruit, root and leaves of Lycium barbarum grown with organic farming techniques using infusion and decoction techniques were evaluated in vitro. The phytochemical contents of the extracts were investigated by spectroscopic and chromatographic techniques. In the study in which five different antioxidant activity methods were used, L. barbarum root decoction showed a strong antioxidant effect in almost all methods. While none of the extracts exerted an inhibitory effect on the alpha-glucosidase enzyme, the leaf infusion of the plant at 2 mg mL-1 concentration caused strong inhibitions especially on pancreatic lipase (62.16 +/- 3.33%) and pancreatic cholesterol esterase (93.98 +/- 0.54%) enzymes compared to the reference compounds. L. barbarum leaf infusion was standardized by RP-HPLC technique on the basis of chlorogenic acid (1.339 +/- 0.056 g 100g-1 dry extract) and quercetin-3-O-glucoside (1.801 +/- 0.042 g 100g-1 dry extract) as markers. The findings displayed that leaf infusions of L. barbarum grown with organic farming techniques could be the source of natural product development studies for hypercholesterolemia and obesity control, and the extract could be standardized using chlorogenic acid and quercetin-3-O-glucoside as